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Scientists efficiently create canine iPSCs using urine-derived cells
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Image source: Shingo Hatoya, Osaka Metropolitan University
OSAKA, Japan – Dog owners may need to learn to appreciate their best friend’s urine. Scientists at Osaka Metropolitan University have devised an efficient, non-invasive, painless method to reprogram canine stem cells from urine samples, bringing furry companions one step closer to veterinary regenerative treatments.
Induced pluripotent stem cells (iPSCs) have been widely used in human reproductive medicine research. As advanced medical care becomes increasingly important for dogs and cats, new therapies utilizing iPSCs are expected to be developed for these companion animals, just as they are for humans. Unfortunately, canine somatic cells reprogram less efficiently than humans, limiting the canine cell types that can be used to generate iPSCs. IPSC induction typically involves the use of feeder cells from different species. However, given the associated risks, it is generally recommended to minimize the xenogeneic component, which implies a need to increase the efficiency of reprogramming various types of canine cells in dogs without the use of feeder cells.
A research team led by Professor Shingo Hatoya and Dr. Masaya Tsukamoto of the Osaka Metropolitan University Graduate School of Veterinary Medicine has identified 6 reprogramming genes that can increase the generation of canine iPSCs approximately 120-fold compared to traditional methods using fibroblasts . iPSCs are generated from urine-derived cells using a non-invasive, simple and painless method. Furthermore, the researchers successfully generated canine iPSCs without feeder cells, a hitherto impossible feat. The team’s goal is to disseminate their findings to the global research community and contribute to the advancement of regenerative medicine and veterinary genetic disease research.
“As a veterinarian, I examine and treat many animals,” Professor Hatoya explained. “However, there are still many diseases that cannot be cured or are not fully understood. In the future, I am committed to continuing to study the differentiation of canine iPSCs into various types of cells and applying them to treat sick dogs, hoping to bring benefits to many animals and their owners. joy.”
The results of their research will be stem cell report December 21, 2023.
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About Ohm
Osaka Metropolitan University is the third largest public university in Japan, formed in 2022 from the merger of Osaka City University and Osaka Prefecture University. OMU upholds the “integration of knowledge” through 11 undergraduate colleges, 1 college and 15 graduate schools. For more research news, visit https://www.omu.ac.jp/en/ or follow us on Twitter: @OsakaMetUniv_enor Facebook.
Magazine
stem cell report
Research methods
Experimental Study
Research Topics
animal
Article title
Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vectors encoding six canine reprogramming factors
Article publication date
December 21, 2023
Conflict of Interest Statement
This research was also funded by Anicom Specialty Medical Institute Inc. Taro Ayabe and Kei Watanabe are employees of Anicom Specialty Medical Institute Inc.
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